Get your free copy of the Nucleofection® Protocol for CRISPR experiments in resting T cells

Discover the new Nucleofection® Protocol for genome editing of resting T cells

View this email as a web page here.     Genome Editing of Resting Primary Human CD4+ T Cells   A step-by-step guide to CRISPR resting CD4+ T cells with tips for higher throughputs and more cell types   Download Protocol       In a new case study, Synthego and Lonza discuss their recent collaboration with Dr. Manuel Albanese and exciting results in gene editing of resting human CD4+ T cells. This case study highlights how to achieve efficient gene editing in CD4+ T cells, overcoming the traditional challenges related to cell viability and maintaining resting state. The corresponding protocol guides you step-by-step through RNP and HDR template delivery in resting primary T cells with Nucleofection® Technology. Of note, further recommendations from Synthego and Lonza on how to adapt the protocol to other cell types and higher throughputs (e.g. 96-well format) are included in a tips and tricks section. Download the protocol: CRISPR Editing Human Primary Resting CD4+ T Cells with RNPs using Nucleofector® Technology.     Download Protocol     This information was prepared by Dr. Manuel Albanese in his personal capacity. The data and any opinions expressed in this presentation are the author's own and do not necessarily reflect the view of his employer.     Home  |   Contact us    Copyright 2023 Lonza. All trademarks belong to Lonza, registered in USA, EU or CH or to third party owners. The information contained herein is believed to be correct. No warranty is made, either expressed or implied. For more details: lonza.com/legal. For further information on how we collect, use and process personal information and your rights in connection therewith, please visit lonza.com/privacy.     Lonza Walkersville, Inc. 8830 Biggs Ford Rd, Walkersville, MD 21793 United States bioscience@lonzabioscience.com     Never miss an email from The Scientist. Click here to add The Scientist to your safe-sender list. The Scientist, 1000 N West Street, Suite 1200,  Wilmington, Delaware, United States, 19801   Toll-Free: (888) 781-0328 | Phone: (705) 528-6888 Email: privacy@the-scientist.com www.the-scientist.com You received this email because you are subscribed to The Scientist Special Offers and Promotions from The Scientist. Click to Unsubscribe | View Privacy Policy Stop receiving email from The Scientist