It’s ok! We’re all a little awkward using bioreactors

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Bioreactors 101
Components of a Bioreactor
Dear Scientist,

We all know different cells have different environmental requirements:
  • Optimal temperature is needed for cell growth and product expression
  • Agitation is critical for good transfer of gases and nutrients without damaging the cells
  • Gas introduction to the culture
  • Monitoring of pH is needed for best cell growth
Read on to learn how bioreactors help control these familiar culture parameters.
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Control agitation by (1) controlling the type of medium flow
Agitation is a way to enhance both nutrient transfer and oxygen transfer to cells for enhanced growth in bioreactors. The impeller used in the bioreactor to influence medium flow is one component helping control agitation. The impeller chosen depends on the cell type where specific impellers can have medium agitated away from the impeller's axis toward the vessel wall or medium agitated up or down along the axis of the impeller or a combination of both.
Control agitation by (2) controlling the medium flow rate
High agitation speed controlled by the motor on the headplate (bioreactor top) connecting to the impeller within the bioreactor can also help with both nutrient and oxygen transfer. The fine control allowed by a bioreactor allows optimization of this spin speed for best growth conditions.
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Gas flow and mixing
Gas is added either via a port with a gas inlet filter on the head plate to the inside headspace of the bioreactor or directly into the medium with a submerged sparger. The sparger comes in different shapes and pore sizes to control the amount and size of the gas bubbles, since bubbles may cause shear stress to certain cells.
Sensors for the most comfortable home for your cells
Sensors for pH, DO, temperature and many more culture components, which are inserted into the headplate or connected to the ports of the headplate, constantly monitor the conditions of the bioreactor to help keep your cells optimally growing.
Steps to run a bioreactor
Bioreactors provide a solid foundation for cell culture experiments by ensuring efficiency, productivity, controllability, monitoring capacity and reproducibility.

Learn to use bioreactors in your applications through Eppendorf!


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