It’s ok! We’re all a little awkward using bioreactors

Review how to get the most out of your cells using bioreactors

View this email as a web page here.   Bioreactors 101     Components of a Bioreactor     Dear Scientist, We all know different cells have different environmental requirements: Optimal temperature is needed for cell growth and product expression Agitation is critical for good transfer of gases and nutrients without damaging the cells Gas introduction to the culture Monitoring of pH is needed for best cell growth Read on to learn how bioreactors help control these familiar culture parameters.     Control agitation by (1) controlling the type of medium flow   Agitation is a way to enhance both nutrient transfer and oxygen transfer to cells for enhanced growth in bioreactors. The impeller used in the bioreactor to influence medium flow is one component helping control agitation. The impeller chosen depends on the cell type where specific impellers can have medium agitated away from the impeller's axis toward the vessel wall or medium agitated up or down along the axis of the impeller or a combination of both.       Control agitation by (2) controlling the medium flow rate   High agitation speed controlled by the motor on the headplate (bioreactor top) connecting to the impeller within the bioreactor can also help with both nutrient and oxygen transfer. The fine control allowed by a bioreactor allows optimization of this spin speed for best growth conditions.       Gas flow and mixing   Gas is added either via a port with a gas inlet filter on the head plate to the inside headspace of the bioreactor or directly into the medium with a submerged sparger. The sparger comes in different shapes and pore sizes to control the amount and size of the gas bubbles, since bubbles may cause shear stress to certain cells.       Sensors for the most comfortable home for your cells   Sensors for pH, DO, temperature and many more culture components, which are inserted into the headplate or connected to the ports of the headplate, constantly monitor the conditions of the bioreactor to help keep your cells optimally growing.       Steps to run a bioreactor   Bioreactors provide a solid foundation for cell culture experiments by ensuring efficiency, productivity, controllability, monitoring capacity and reproducibility. Learn to use bioreactors in your applications through Eppendorf!   Take A Closer Look     Eppendorf North America   Eppendorf North America • 100 Crossing Blvd, Suite 110 • Framingham, MA, 01702 info@eppendorf.com • Phone: 800 645 3050 • www.eppendorf.com               ©  Eppendorf North America     Imprint     Legal Notice & Privacy Policy     Never miss an email from The Scientist. Click here to add The Scientist to your safe-sender list. The Scientist, 1000 N West Street, Suite 1200,  Wilmington, Delaware, United States, 19801   Toll-Free: (888) 781-0328 | Phone: (705) 528-6888 Email: privacy@the-scientist.com www.the-scientist.com You received this email because you are subscribed to The Scientist Special Offers and Promotions from The Scientist. Click to Unsubscribe | View Privacy Policy Stop receiving email from The Scientist